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Registro Completo |
Biblioteca(s): |
Embrapa Rondônia. |
Data corrente: |
28/11/2017 |
Data da última atualização: |
29/11/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, M. R. A. dos; SOUZA, P. G. |
Afiliação: |
MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia. |
Título: |
Evoluation of the proleferation of dedifferentiated cells of yellow bell pepper (Caapsicum annuum cv. Pimentao amarelo). |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
International Journal of Development Research, v. 7, n. 8, p.14566-14569, Aug. 2017. |
Idioma: |
Inglês |
Conteúdo: |
Capsicum annuum is notable for its secondary metabolites, which can be of great interest for use in agriculture as alternatives to agrochemicals. Cell suspensions have been used for in vitro largescale production of secondary metabolites. The aim of this study was to dedifferentiate leaf cells of Capsicum annuum cv. Pimentão Amarelo (yellow bell pepper) into calluses, and to study their growth pattern, focusing on the deceleration phase, when they can be subcultured to establish cell suspensions. Leaf explants were inoculated on MS medium supplemented with the growth regulators 2,4-D (0, 4.52, 9.05 and 18.10 µM) and BA (0, 4.44, 8.88 and 17.76 µM) in factorial combinations. Callus formation was evaluated weekly for 42 days, by assessing the number of callus induced per treatment and the weight of the explants. The culture medium supplemented with 4.52 µM 2,4-D and 4.44 µM BA resulted in the highest callus induction and weight of callus. For the identification of the growth curve the explants were submitted to this combination and in the subsequent 42 days, every seven days, the weight of the calluses was periodically measured. The growth curve has a sigmoid pattern and the deceleration phase begins 21 days after inoculation. |
Palavras-Chave: |
Calluses; Solanacease. |
Thesaurus Nal: |
secondary metabolites. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/167681/1/2017-IJDR-Yellow-Pepper.pdf
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Marc: |
LEADER 01827naa a2200169 a 4500 001 2080930 005 2017-11-29 008 2017 bl uuuu u00u1 u #d 100 1 $aSANTOS, M. R. A. dos 245 $aEvoluation of the proleferation of dedifferentiated cells of yellow bell pepper (Caapsicum annuum cv. Pimentao amarelo).$h[electronic resource] 260 $c2017 520 $aCapsicum annuum is notable for its secondary metabolites, which can be of great interest for use in agriculture as alternatives to agrochemicals. Cell suspensions have been used for in vitro largescale production of secondary metabolites. The aim of this study was to dedifferentiate leaf cells of Capsicum annuum cv. Pimentão Amarelo (yellow bell pepper) into calluses, and to study their growth pattern, focusing on the deceleration phase, when they can be subcultured to establish cell suspensions. Leaf explants were inoculated on MS medium supplemented with the growth regulators 2,4-D (0, 4.52, 9.05 and 18.10 µM) and BA (0, 4.44, 8.88 and 17.76 µM) in factorial combinations. Callus formation was evaluated weekly for 42 days, by assessing the number of callus induced per treatment and the weight of the explants. The culture medium supplemented with 4.52 µM 2,4-D and 4.44 µM BA resulted in the highest callus induction and weight of callus. For the identification of the growth curve the explants were submitted to this combination and in the subsequent 42 days, every seven days, the weight of the calluses was periodically measured. The growth curve has a sigmoid pattern and the deceleration phase begins 21 days after inoculation. 650 $asecondary metabolites 653 $aCalluses 653 $aSolanacease 700 1 $aSOUZA, P. G. 773 $tInternational Journal of Development Research$gv. 7, n. 8, p.14566-14569, Aug. 2017.
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1. | | SIQUEIRA, M. M. B.; CARVALHO, M. T. de M.; CALIL, F. N.; MACHADO, P. L. O. de A.; FERREIRA, C. M.; FREITAS, F. M. C.; RIZZO, P. V.; CAN, E.; RAMSDEN, S.; BARROSO NETO, J.; OLIVEIRA, S. M.; FERNANDES, J. O.; PACHECO, A. R.; MADARI, B. E. Carbon balance of a small dairy farm with integrated livestock-forest system in Goiás, Central West region of Brazil. In: CONGRESO INTERNACIONAL DE SISTEMAS SILVOPASTORILES, 12.; CONGRESO DE LA RED GLOBAL DE SISTEMAS SILVOPASTORILES, 2.; IV SEMINARIO NACIONAL DE SISTEMAS SILVOPASTORILES, 4., 2023, Montevideo; CONGRESO NACIONAL SISTEMAS SILVOPASTORILES, 5., 2023, Buenos Aires. Sistemas silvopastoriles: hacia una diversificación sostenible. Cali: CIPAV, 2023. p. 686-690.Tipo: Artigo em Anais de Congresso |
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2. | | MADARI, B. E.; MATOS, P. S.; OLIVEIRA, J. M.; CARVALHO, M. T. de M.; SILVEIRA, A. L. R.; MACHADO, P. L. O. de A.; ARAUJO, W. A.; SIQUEIRA, M. M. B.; FERRARESI, T. M.; FREITAS, F. M. C.; RIZZO, P. V.; SILVA, R. R.; PACHECO, A. Carbono orgánico del suelo en cultivos-ganadería-silvicultura en un estudio de caso de la región centro-oeste de Brasil. In: CONGRESO INTERNACIONAL DE SISTEMAS SILVOPASTORILES, 12.; CONGRESO DE LA RED GLOBAL DE SISTEMAS SILVOPASTORILES, 2.; IV SEMINARIO NACIONAL DE SISTEMAS SILVOPASTORILES, 4., 2023, Montevideo; CONGRESO NACIONAL SISTEMAS SILVOPASTORILES, 5., 2023, Buenos Aires. Sistemas silvopastoriles: hacia una diversificación sostenible. Cali: CIPAV, 2023. p. 547-560.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Florestas; Embrapa Gado de Leite. |
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3. | | MADARI, B. E.; CARVALHO, M. T. de M.; OLIVEIRA, J. de M.; SILVEIRA, A. L. R. da; FREITAS, F. M. C.; KOAKUZU, S. N.; SIQUEIRA, M. M. de B.; ARAÚJO, W. A. de; PACHECO, A. R.; MACHADO, P. L. O. de A. Soil carbon stocks at 3 and 11 years of an integrated crop-livestock-forestry system on a clayey Ferralsol in the brazilian savanna. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS: 100% DIGITAL, 2., 2021. WCCLF 2021 proceedings. Campo Grande, MS: Embrapa Gado de Corte, 2021. p. 72-78.Tipo: Artigo em Anais de Congresso |
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